Human Hypoxia-inducible Factor 1Α, HIF-1A ELISA Kit

Overview:

Target Protein: Hypoxia-Inducible Factor 1 alpha, HIF1A

Size: 48T, 96T

Assay Type: Sandwich

One-Step: -

Sensitivity: 0.01ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX1 seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, is required for induction of glycolysis in monocytes and the consequent proinflammatory state (PubMed: 32697943). In monocytes, induces expression of ACE2 and cytokines such as IL1B, TNF, IL6, and interferons (PubMed: 32697943). Promotes human coronavirus SARS-CoV-2 replication and monocyte inflammatory response (PubMed: 32697943).

HIF1A Information:

UniProt Accession: Q16665

Synonyms: ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;bHLHe78;Class E basic helix-loop-helix protein 78;HIF 1A;HIF1A;HIF1-alpha;HIF-1-alpha;Hypoxia-inducible factor 1-alpha;Member of PAS protein 1;PAS domain-containing protein 8

References:

• Metabolic/hypoxial axis predicts tamoxifen resistance in breast cancer Journal: Sci Rep. 2022 Sep 27;12(1):16118.
• New Avenues to Explore in SARS-CoV-2 Infection: Both TRIM25 and TRIM56 Positively Correlate with VEGF, GAS6, and sAXL in COVID-19 Patients Journal: Viral Immunol. 2022 Dec;35(10):690-699.
• Evaluation of potential tumor markers that may predict neoadjuvant treatment efficiency in rectal cancer Journal: Arslan, Fatma Demet, Kocak, Ayse, Aydın, Cengiz, Pala, Emel Ebru, Oncel, Dilek, Diniz, Gulden, Kaya, Tayfun, Ugurlu, Levent, Degirmenci, Mustafa, Ozkan, Bulent, Soysal, Yasemin and Said, Harun Muayad. "Evaluation of potential tumor markers that may predict neoadjuvant treatment efficiency in rectal cancer" Turkish Journal of Biochemistry, vol. 46, no. 4, 2021, pp. 445-454.
• Serum HIF-1α Levels, miR-210 Expressions, and Reactive Oxygen Species Levels in Early Abortion and Normal Pregnancy Journal: Serudji J, Irawati N, Mose JC, Ali H, Yusrawati Y. Serum HIF-1α Levels, miR-210 Expressions, and Reactive Oxygen Species Levels in Early Abortion and Normal Pregnancy. Open Access Maced J Med Sci [Internet]. 2022 Aug. 4 [cited 2023 Nov. 7];10(B):1779-83.
• The role of HIF-1α in COVID-19 pathogenesis and its correlation with IL-6 and other inflammation parameters Journal: Hawzhin Khalid, Soheila Asadi, Ayad Palani et al. The role of HIF-1α in COVID-19 pathogenesis and its correlation with IL-6 and other inflammation parameters, 05 January 2023, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-2444358/v1]

Supplier: BT-Labs