Human Interferon Regulatory Factor 3, IRF3 ELISA Kit

Overview:

Target Protein: Interferon Regulatory Factor 3, IRF3

Size: 48T, 96T

Assay Type: Sandwich

One-Step: -

Sensitivity: 0.054ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed: 22394562, PubMed: 25636800, PubMed: 27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed: 11846977, PubMed: 16846591, PubMed: 16979567, PubMed: 20049431, PubMed: 32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed: 16846591, PubMed: 16979567, PubMed: 20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed: 22394562, PubMed: 25636800, PubMed: 27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed: 16154084, PubMed: 27302953, PubMed: 33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed: 16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed: 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed: 33440148).

IRF3 Information:

UniProt Accession: Q14653

Synonyms: Interferon regulatory factor 3;IRF 3;IRF3;IRF-3

Supplier: BT-Labs