Human Neutrophil Gelatinase-associated Lipocalin, NGAL ELISA Kit

Overview:

Target Protein: Neutrophil Gelatinase-Associated Lipocalin, LCN2

Size: 48T, 96T

Assay Type: Sandwich

One-Step: -

Sensitivity: 2.01ng/ml

Detection range: 5-600ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (PubMed: 12453413, PubMed: 27780864, PubMed: 20581821). Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin (PubMed: 27780864). Can also bind siderophores from M.tuberculosis (PubMed: 15642259, PubMed: 21978368).

LCN2 Information:

UniProt Accession: P80188

Synonyms: 25 kDa alpha-2-microglobulin-related subunit of MMP-9;LCN 2;LCN2;Lipocalin-2;Neutrophil gelatinase-associated lipocalin;NGAL;Oncogene 24p3;p25;Siderocalin;

References:

• Plasma Neutrophil Gelatinase-Associated Lipocalin as a Marker for Prediction of 3-Month Graft Survival after Kidney Transplantation
• Journal: Int J Organ Transplant Med. 2017; 8(1): 17–27.
• Comparison of Two Different Inhalation Anesthetics on Grafted Kidney Function in Patients Undergoing Renal Transplantation Surgery: Desflurane or Sevoflurane?
• Journal: Transplant Proc. 2017 Apr;49(3):448-453.
• Predictive Values of Urinary Interleukin 18 and Neutrophil Gelatinase-Associated Lipocalin for Delayed Graft Function Diagnosis in Kidney Transplantation
• Journal: Iran J Pathol. 2016 Fall;11(4):391-398.
• Effect of Hypertonic Saline 5% on Early Graft Function and Urinary Interleukin 18 and Neutrophil Gelatinase-Associated Lipocalin in Deceased-Donor Kidney Transplantation
• Journal: Iran J Pharm Res. 2017 Fall;16(4):1583-1592.
• Serum neutrophil gelatinase-associated lipocalin concentrations are significantly associated with the severity of COVID-19 in pregnant patients
• Journal: Saudi Med J. 2022 Jun;43(6):559-566.
• Urine neutrophil gelatinase-associated lipocalin as a biomarker of acute kidney injury and prognosis in decompensated chronic liver disease: A prospective study
• Journal: Indian J Gastroenterol. 2023 Feb;42(1):106-117.
• Cotreatment with Furosemide and Hypertonic Saline Decreases Serum Neutrophil Gelatinase-associated Lipocalin (NGAL) and Serum Creatinine Concentrations in Traumatic Brain Injury: A Randomized, Single-Blind Clinical Trial
• Journal: Iran J Pharm Res. Summer 2018;17(3):1130-1140.
• Trial of Furosemide to Prevent Acute Kidney Injury in Critically Ill Children: A Double-Blind, Randomized, Controlled Trial
• Journal: Indian J Pediatr. 2021 Nov;88(11):1099-1106.
• Incidence, Risk Factors, the Role of Plasma NGAL and Outcome of Contrast-Induced Acute Kidney Injury in Critically Ill Children
• Journal: Indian J Pediatr. 2021 Jan;88(1):34-40.
• THE RISK OF ACUTE KIDNEY INJURY AFTER ELECTIVE VERSUS EMERGENCY CORONARY INTERVENTION
• Journal: Wiad Lek. 2022;75(12):3010-3017.

Supplier: BT-Labs