Human Nuclear Factor Erythroid 2-related Factor 2, NRF2 ELISA Kit

Overview:

Target Protein: Nuclear Factor Erythroid 2-Related Factor 2, NFE2L2

Size: 48T, 96T

Assay Type: Sandwich

One-Step: -

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles (PubMed: 11035812, PubMed: 19489739, PubMed: 29018201, PubMed: 31398338). In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex (PubMed: 11035812, PubMed: 15601839, PubMed: 29018201). In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes (PubMed: 19489739, PubMed: 29590092). The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (PubMed: 20452972). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (PubMed: 7937919).

NFE2L2 Information:

UniProt Accession: Q16236

Synonyms: HEBP1;NFE2L2;NFE2-related factor 2;NF-E2-related factor 2;Nrf-2;Nuclear factor erythroid 2-related factor 2;Nuclear factor, erythroid derived 2, like 2

Supplier: BT-Labs