Human Toll-like Receptor 4, TLR4 ELISA Kit

Overview:

Target Protein: Toll-Like Receptor 4, TLR4

Size: 48T, 96T

Assay Type: Sandwich

One-Step: -

Sensitivity: 0.027ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed: 27022195). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed: 9237759, PubMed: 10835634, PubMed: 27022195,PubMed: 21393102). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific (PubMed: 20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed: 15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed: 23880187). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor (PubMed: 16622205, PubMed: 10835634, PubMed: 15809303, PubMed: 17478729, PubMed: 20037584, PubMed: 20711192, PubMed: 23880187, PubMed: 27022195, PubMed: 9237759). Activated by the signaling pathway regulator NMI which acts as damage-associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (PubMed: 29038465).

TLR4 Information:

UniProt Accession: O00206

Synonyms: CD antigen CD284;hToll;TLR 4;TLR4;Toll-like receptor 4

Supplier: BT-Labs