Mouse Tumor Necrosis Factor Αlpha, TNF-A ELISA Kit

Overview:

Target Protein: Tumor Necrosis Factor alpha (TNFA)

Size: 48T, 96T

Assay Type: Sandwich

One-Step: -

Sensitivity: 2.49ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed: 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

TNFA Information:

UniProt Accession: P06804

Synonyms: Cachectin;C-domain 1;C-domain 2;ICD1;ICD2;Intracellular domain 1;Intracellular domain 2;N-terminal fragment;NTF;TNF A;TNF alpha;TNFA;TNF-a;TNF-alpha;Tumor necrosis factor;Tumor necrosis factor ligand superfamily member 2;Tumor necrosis factor, membrane form;Tumor necrosis factor, soluble form

References:

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Supplier: BT-Labs