Rat Interleukin 10, IL-10 ELISA Kit

Overview:

Target Protein: Interleukin 10 (IL10)

Size: 48T, 96T

Assay Type: Sandwich

One-Step: +

Sensitivity: 1.51pg/ml

Detection range: 3-900pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Storage: 2 - 8°C

Assay Time: 1:30 h

Detailed Information:

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity).

IL10 Information:

UniProt Accession: P29456

Synonyms: CSIF;Cytokine synthesis inhibitory factor;IL 10;IL10;IL-10;Interleukin-10

References:

• Evaluation of anti-amnesic effect of Conyza bonariensis in ratsJournal: Indian J Pharmacol. 2022 Mar-Apr;54(2):102-109.
• Synergistic Effect of Omega-3 and Probiotic Supplementation on Preventing Ligature-Induced PeriodontitisJournal: Probiotics Antimicrob Proteins. 2022 Feb;14(1):114-120
• The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in RatsJournal: Turk Neurosurg. 2023;33(1):162-170.
• Regeneration of Salivary Gland Defects of Diabetic Wistar Rats Post Human Dental Pulp Stem Cells Intraglandular Transplantation on Acinar Cell Vacuolization and Interleukin-10 Serum LevelJournal: Pesqui. Bras. Odontopediatria Clín. Integr. 19 • 2019 •
• Anti-Inflammatory Effect of Crude Momordica charantia L. Extract on 2,4,6-Trinitrobenzene Sulfonic Acid-Induced Colitis Model in Rat and the Bioaccessibility of its Carotenoid ContentJournal: J Med Food. 2020 Jun;23(6):641-648.
• Investigation of the Gastroprotective Effect of Betaine-Homocysteine Homeostasis on Oxidative Stress, Inflammation and Apoptosis in Ethanol-Induced Ulcer ModelJournal: J Invest Surg. 2022 Nov-Dec;35(11-12):1806-1817.
• Boric Acid Alleviates Gastric Ulcer by Regulating Oxidative Stress and Inflammation-Related Multiple Signaling PathwaysJournal: Biol Trace Elem Res. 2023 Aug 22.
• Prophylactic and Therapeutic Efficacy of Boric Acid on Lipopolysaccharide-Induced Liver and Kidney Inflammation in RatsJournal: Biol Trace Elem Res. 2023 Nov 1.
• Moderate and high-intensity interval training protect against diabetes-induced modulation of hepatic CD86 and CD206 expression associated with the amelioration of insulin resistance and inflammation in ratsJournal: Immunobiology. 2023 Sep 13;228(6):152745.
• Costunolide prevents renal ischemia-reperfusion injury in rats by reducing autophagy, apoptosis, inflammation, and DNA damageJournal: Iran J Basic Med Sci. 2023;26(10):1168-1176.

Supplier: BT-Labs